Batch-Type Detection Cell Using a Peroxyoxalate Chemiluminescence System for Capillary Electrophoresis.

Improvement of a capillary electrophoresis-chemiluminescence detection system for using a polyacrylamide-coated capillary.

Capillary electrophoresis-chemiluminescence detection system equipped with a consecutive sample-injection device.

We have developed a consecutive sample-injection device for capillary electrophoresis, which comprises one four-way cock, two syringe pumps, and an interface part taking advantage of two three-way Teflon joints. Sample introduction into the capillary is made hydrodynamically by pressure, caused by the flow of the sample solution at the tip of the capillary inlet. We combined the injection devic...

Chemiluminescence Properties of Peroxyoxalate System in Organic Solvents

Peroxyoxalate system includes an oxalate ester, peroxide and a fluorescer, The energy resulted from the reaction of oxalate ester and peroxide is transferred to fluorescer and cause it to fluorsc. The effect of reactants chemical structure, their concentration, solvent media (polarity and viscosity) and the temperature, on the intensity of the illuminated light with respect to time have bee...

Determination of phenothiazines in pharmaceutical formulations and human urine using capillary electrophoresis with chemiluminescence detection.

A CE instrument coupled with chemiluminescence (CL) detection was designed for the determination of promethazine hydrochloride (PTH) and promazine hydrochloride (PMH) in real samples. An important enhancement of the CL emission of luminol with potassium ferricyanide was observed in the presence of these phenothiazines; so this system was selected for their detection after CE separation. Paramet...

Introducing a New and Simple Protocol for Capillary Electrophoresis of Cell Free Fetal Double Stranded DNA

Isolation of cell free fetal DNA (cffDNA) from maternal serum usually leads to very low concentrations of DNA impeding further resolving through conventional methods of electrophoresis. Although several protocols have been described for capillary electrophoresis (CE) of double stranded DNA, they usually need using special polymers or coated capillaries which degrade over time. Herein, we propos...